RESUMO
Human and animal dermatophytoses are most commonly treated with systemic antifungal drugs such as itraconazole (ITZ) and terbinafine (TRF). The antifungal susceptibility of Nannizia fulva, however, remains poorly documented. In the present study, we investigated the in vitro susceptibility of N. fulva to ITZ and TRF using the CLSI M38-A2 test. The mean MICs for the 12 tested strains were 0.6542 mg/L (range: 0.0625-1 mg/L) for ITZ and 0.15625 mg/L (range: <0.003125-0.5 mg/L) for TRF. These results indicate that ITZ and TRF at standard veterinary doses should be efficacious against N. fulva.
Assuntos
Antifúngicos/farmacologia , Arthrodermataceae/isolamento & purificação , Itraconazol/farmacologia , Terbinafina/farmacologia , Tinha/microbiologia , Doenças dos Animais/tratamento farmacológico , Doenças dos Animais/microbiologia , Animais , Relação Dose-Resposta a Droga , Farmacorresistência Fúngica , Humanos , Itraconazol/uso terapêutico , Japão , Terbinafina/uso terapêutico , Tinha/tratamento farmacológico , Tinha/veterináriaRESUMO
In the case presented here, we describe the isolation of an azole-resistant strain of M. pachydermatis from a canine Malassezia dermatitis. The isolate (NUBS18001) from this case exhibited a minimum inhibitory concentration (MIC) of 320 µg/ml to itraconazole (ITZ) by broth microdilution (BM) assay, >32 µg/ml to ITZ by E-test, and >32 µg/ml to KTZ by E-test. Synergistic effects between FK506 and ITZ in the azole-resistant strain was evaluated using the microdilution checker-board method. The ITZ-resistant strain exhibited MICs of 320 µg/mL of ITZ alone and 5 µg/ml of FK506 alone; the addition of FK506 attenuated the ITZ MIC to 2.5 µg/ml, yielding an ITZ FICI value of 0.507. This result suggested that the combination of ITZ and FK506 exerted an additive effect against the ITZ-resistant strain. To understand the other mechanism inferred to be present in our multi-azole-resistant strain, we sequenced the ERG11 gene from this isolate, and detected missense mutations (A412G and C905T) in the sequence of the ERG11 open reading frame (ORF). To the best of our knowledge, this work is the first report that a multi-azole-resistant M. pacydermatis strain contains mutations in ERG11.